S2407

Sigma

 

Monoclonal Anti-S-100 (α-Subunit) antibody produced in mouse

clone SH-A1, ascites fluid

MDL number:MFCD00162834

Description

Immunogenbovine brain S-100a
SpecificityRecognizes an epitope located on the α chain (i.e. in S-100ao and S-100a) but not on the β chain (S-100b) of S-100. In ELISA, reactivity of the antibody with S-100 α-subunit is independent of Ca2+ ion. The product is also reactive in dot blot assay using denatured-reduced preparations, and in certain immunohistochemical techniques. Cross-reactivity has been observed with S-100 of human and bovine, but not with other members of the EF-hand family such as calmodulin, parvalbumin, intestinal calcium-binding protein and myosin light chain. The product is useful in the quantification of S-100ao and S-100a, applying competitive ELISA.

Properties

antibody formascites fluid
cloneSH-A1 (monoclonal)
contains15 mM sodium azide
species reactivityhuman, bovine
application(s)capture ELISA: suitable
 dot blot: suitable using denatured-reduced preparations
 indirect ELISA: 1:1,000 using bovine brain S-100
 microarray: suitable
isotypeIgG1
Gene Informationhuman ... S100A1(6271)
shipped indry ice
storage temp.−20°C

Safety

WGK GermanyNWG

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References

referenceWalter, I., and Miller, I. Histochem. J. 28, 671, (1996) Abstract
 Aebi, U., et al., Distinct subcellular location of the Ca2+-binding protein S100A1 differentially modulates Ca2+-cycling in ventricular rat cardiomyocytes. J. Cell Sci. 118, 421-431, (2005) Abstract
 Acklin, C., et al., Ca2+ -dependent interaction of S100A1 with the sarcoplasmic reticulum Ca2+ -ATPase2a and phospholamban in the human heart. Biochem. Biophys. Res. Commun. 306, 550-557, (2003) Abstract
 Castagna, C., and Viglietti-Panzica, C., Protein S100 immunoreactivity in glial cells and neurons of the Japanese quail brain. J. Chem. Neuroanat. 25, 195-212, (2003) Abstract